| Effects of Simulated Microgravity on Embryonic Stem Cells | |
| Wang, Yulan; An, Lili; Jiang, Yuanda; Hang, Haiying; Wang, YL (reprint author), Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100080, Peoples R China. | |
| Department | 空间技术部 |
| Source Publication | PLOS ONE
 |
| 2011 | |
| Volume | 6Issue:12Pages:e29214 |
| ISSN | 1932-6203 |
| Language | 英语 |
| Abstract | There have been many studies on the biological effects of simulated microgravity (SMG) on differentiated cells or adult stem cells. However, there has been no systematic study on the effects of SMG on embryonic stem (ES) cells. In this study, we investigated various effects (including cell proliferation, cell cycle distribution, cell differentiation, cell adhesion, apoptosis, genomic integrity and DNA damage repair) of SMG on mouse embryonic stem (mES) cells. Mouse ES cells cultured under SMG condition had a significantly reduced total cell number compared with cells cultured under 1 g gravity (1G) condition. However, there was no significant difference in cell cycle distribution between SMG and 1G culture conditions, indicating that cell proliferation was not impaired significantly by SMG and was not a major factor contributing to the total cell number reduction. In contrast, a lower adhesion rate cultured under SMG condition contributed to the lower cell number in SMG. Our results also revealed that SMG alone could not induce DNA damage in mES cells while it could affect the repair of radiation-induced DNA lesions of mES cells. Taken together, mES cells were sensitive to SMG and the major alterations in cellular events were cell number expansion, adhesion rate decrease, increased apoptosis and delayed DNA repair progression, which are distinct from the responses of other types of cells to SMG.; There have been many studies on the biological effects of simulated microgravity (SMG) on differentiated cells or adult stem cells. However, there has been no systematic study on the effects of SMG on embryonic stem (ES) cells. In this study, we investigated various effects (including cell proliferation, cell cycle distribution, cell differentiation, cell adhesion, apoptosis, genomic integrity and DNA damage repair) of SMG on mouse embryonic stem (mES) cells. Mouse ES cells cultured under SMG condition had a significantly reduced total cell number compared with cells cultured under 1 g gravity (1G) condition. However, there was no significant difference in cell cycle distribution between SMG and 1G culture conditions, indicating that cell proliferation was not impaired significantly by SMG and was not a major factor contributing to the total cell number reduction. In contrast, a lower adhesion rate cultured under SMG condition contributed to the lower cell number in SMG. Our results also revealed that SMG alone could not induce DNA damage in mES cells while it could affect the repair of radiation-induced DNA lesions of mES cells. Taken together, mES cells were sensitive to SMG and the major alterations in cellular events were cell number expansion, adhesion rate decrease, increased apoptosis and delayed DNA repair progression, which are distinct from the responses of other types of cells to SMG. |
| Indexed By | SCI |
| Funding Project | 中国科学院空间科学与应用研究中心 |
| Document Type | 期刊论文 |
| Identifier | http://ir.nssc.ac.cn/handle/122/3007 |
| Collection | 空间技术部 |
| Corresponding Author | Wang, YL (reprint author), Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100080, Peoples R China. |
| Recommended Citation GB/T 7714 | Wang, Yulan,An, Lili,Jiang, Yuanda,et al. Effects of Simulated Microgravity on Embryonic Stem Cells[J]. PLOS ONE,2011,6(12):e29214. |
| APA | Wang, Yulan,An, Lili,Jiang, Yuanda,Hang, Haiying,&Wang, YL .(2011).Effects of Simulated Microgravity on Embryonic Stem Cells.PLOS ONE,6(12),e29214. |
| MLA | Wang, Yulan,et al."Effects of Simulated Microgravity on Embryonic Stem Cells".PLOS ONE 6.12(2011):e29214. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| 2011612e29214.pdf（513KB） | 开放获取 | CC BY-NC-SA | Application Full Text | |||
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