NSSC OpenIR  > 空间技术部
Effects of Simulated Microgravity on Embryonic Stem Cells
Wang, Yulan; An, Lili; Jiang, Yuanda; Hang, Haiying; Wang, YL (reprint author), Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100080, Peoples R China.
Department空间技术部
Source PublicationPLOS ONE
2011
Volume6Issue:12Pages:e29214
ISSN1932-6203
Language英语
AbstractThere have been many studies on the biological effects of simulated microgravity (SMG) on differentiated cells or adult stem cells. However, there has been no systematic study on the effects of SMG on embryonic stem (ES) cells. In this study, we investigated various effects (including cell proliferation, cell cycle distribution, cell differentiation, cell adhesion, apoptosis, genomic integrity and DNA damage repair) of SMG on mouse embryonic stem (mES) cells. Mouse ES cells cultured under SMG condition had a significantly reduced total cell number compared with cells cultured under 1 g gravity (1G) condition. However, there was no significant difference in cell cycle distribution between SMG and 1G culture conditions, indicating that cell proliferation was not impaired significantly by SMG and was not a major factor contributing to the total cell number reduction. In contrast, a lower adhesion rate cultured under SMG condition contributed to the lower cell number in SMG. Our results also revealed that SMG alone could not induce DNA damage in mES cells while it could affect the repair of radiation-induced DNA lesions of mES cells. Taken together, mES cells were sensitive to SMG and the major alterations in cellular events were cell number expansion, adhesion rate decrease, increased apoptosis and delayed DNA repair progression, which are distinct from the responses of other types of cells to SMG.; There have been many studies on the biological effects of simulated microgravity (SMG) on differentiated cells or adult stem cells. However, there has been no systematic study on the effects of SMG on embryonic stem (ES) cells. In this study, we investigated various effects (including cell proliferation, cell cycle distribution, cell differentiation, cell adhesion, apoptosis, genomic integrity and DNA damage repair) of SMG on mouse embryonic stem (mES) cells. Mouse ES cells cultured under SMG condition had a significantly reduced total cell number compared with cells cultured under 1 g gravity (1G) condition. However, there was no significant difference in cell cycle distribution between SMG and 1G culture conditions, indicating that cell proliferation was not impaired significantly by SMG and was not a major factor contributing to the total cell number reduction. In contrast, a lower adhesion rate cultured under SMG condition contributed to the lower cell number in SMG. Our results also revealed that SMG alone could not induce DNA damage in mES cells while it could affect the repair of radiation-induced DNA lesions of mES cells. Taken together, mES cells were sensitive to SMG and the major alterations in cellular events were cell number expansion, adhesion rate decrease, increased apoptosis and delayed DNA repair progression, which are distinct from the responses of other types of cells to SMG.
Indexed BySCI
Funding Project中国科学院空间科学与应用研究中心
Document Type期刊论文
Identifierhttp://ir.nssc.ac.cn/handle/122/3007
Collection空间技术部
Corresponding AuthorWang, YL (reprint author), Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100080, Peoples R China.
Recommended Citation
GB/T 7714
Wang, Yulan,An, Lili,Jiang, Yuanda,et al. Effects of Simulated Microgravity on Embryonic Stem Cells[J]. PLOS ONE,2011,6(12):e29214.
APA Wang, Yulan,An, Lili,Jiang, Yuanda,Hang, Haiying,&Wang, YL .(2011).Effects of Simulated Microgravity on Embryonic Stem Cells.PLOS ONE,6(12),e29214.
MLA Wang, Yulan,et al."Effects of Simulated Microgravity on Embryonic Stem Cells".PLOS ONE 6.12(2011):e29214.
Files in This Item: Download All
File Name/Size DocType Version Access License
2011612e29214.pdf(513KB) 开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Yulan]'s Articles
[An, Lili]'s Articles
[Jiang, Yuanda]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Yulan]'s Articles
[An, Lili]'s Articles
[Jiang, Yuanda]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Yulan]'s Articles
[An, Lili]'s Articles
[Jiang, Yuanda]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 2011612e29214.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.